Cytotoxic and Osteoblast Differentiation Induction Properties of Crude Polar Extract of Piper sarmentosum leaves

Piper sarmentosum or ‘kaduk’ is a well-known herb plant in Malaysia. Its extracts were found to exhibit bone-protective effect against osteoporotic rats. Our study aim morphologically observe the of P. ethyl acetate extract on differentiation human peripheral blood stem cells (PBSCs) into osteoblasts. (1-900 µg/mL) prepared using 1% dimethyl sulfoxide (DMSO) used cytotoxicity assay. Then, assay was performed concentrations 1-50 µg/mL. The untreated acted as negative control, while cultured 50 µg/mL ascorbic acid and 10 mM β-glycerophosphate positive control. proliferation capacity analyzed Trypan Blue exclusion method, PBSCs observed von Kossa staining ALP gene expression analysis. result showed decrease cells’ viability dose-dependent manner during After 14 days differentiation, constant rate could be treated an increase proliferation. mineralization extract-treated significant differences (p<0.05) compared also upregulated day 0. In conclusion, capable induce osteoblast cell potential tissue regeneration